LOCUS       40924_23849       12879 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB752 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12879)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12879)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12879)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1891
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    1901..2025
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2062..2092
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2146..2802
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3147..3449
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3493..3617)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3643..3895
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3929..3945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3953..3969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3977..4007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4022..4043)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4079..4331)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4369..5664)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61281.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4369..5664)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5716..5895)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6401..6425)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6946..7734
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7983..8571
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8757..8897)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9241..9435)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9504..10574)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11006..11632)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg agtaaaggag aagaactttt
     1201 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1261 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1321 cactactgga aaactacctg ttccatggcc aacacttgtt actactttcg cttacggtgt
     1381 gcagtgcttc tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat
     1441 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac
     1501 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat
     1561 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact tcaactccca
     1621 caacgtgtac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     1681 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     1741 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1801 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1861 gattacacat ggcatggatg aactatacaa agctgttatc acaagtttgt acaaaaaagc
     1921 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     1981 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc ccctcgaggg
     2041 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2101 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2161 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     2221 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2281 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2341 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     2401 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2461 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2521 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     2581 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2641 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2701 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2761 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     2821 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     2881 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     2941 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3001 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3061 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3121 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3181 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3241 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     3301 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     3361 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     3421 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     3481 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     3541 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     3601 ttcttgtaca aagtggtgat aacctagagc tcgaatttcc ccgatcgttc aaacatttgg
     3661 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     3721 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     3781 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     3841 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     3901 ttggttccgg aaccaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4021 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga
     4081 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     4141 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     4201 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     4261 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     4321 tttgaacgat cggggaaatt cgcctcgagt gacttctctg attgcagact atttatacca
     4381 accagcaaga aagtgtcgaa gtatgaagca gaaggcgcag gctagagcct ggaaaacgag
     4441 aagatggatg caaagagact tttctgattt aggaccaaac aacctcaagt aaacattgac
     4501 gagtgtccca tcttttgtgc cggtcagtaa tccggtagca ggatcatacc ttgggagacg
     4561 atggcgcgga catttcacaa tcccagcaag ctgaggaagc gataatagaa ggttcagaac
     4621 ttgaggaaga aagaagatga ggagggtttc actgaagtga cccagaatac caacaactgc
     4681 catagtcatt ccggcgaaga ctgtgtaagt gtctccaaca aaaactgaag aaggatacca
     4741 attgtaagca agcattgcca aggatgtggc catcagcggt tgagtaagaa atatcgagaa
     4801 ggcatgagct tggtgatact cgggatcaac agatgctccg atttgcataa cgttgtgtat
     4861 caggatagca gcggcaataa caactgtttg accgatttca aggccattca gaccagcgtg
     4921 gatgttaata gagtttgtac aaaagacagc aagtagcccc atatataact tatatatcct
     4981 tcctagatta agcacttcca agccaatata agcaacaaga ggttttggta taacaatagt
     5041 tgtatgacca gcataagcca tcaaaagtgg aagggtagca aaagatggca ggacaagttt
     5101 tactctccaa ggcacatcaa ggacatcatc tacaaatcca agcaaaatca tgaagcaaat
     5161 agatgctagt gctgcattat actccacaag ccaattagaa tcttcagtga agttaaaata
     5221 ctgaaatatt atcgccacga tcaaaaagac aataccaaca acaataccca atgactctgg
     5281 cactttaata tctccttgag gcgtgcctct tttgttgata tcaaaaccaa acatgttacg
     5341 tctcagaacg tatctggcag taacaggaat cattttgaga gtgacgaaaa acccaaccaa
     5401 gctaagcccc gcgttaatta gaatcgatcg cttgagttca ttatcaacct tgtagtgaaa
     5461 acagagaagg tatagataga gcgaacagag aagagtagag atcacgaaga tcactcctaa
     5521 tttcggcggc gcgagacgaa attcctcacc ggaagcagaa accgtctttc gggtcatctt
     5581 ctgctccgat ggcgctgagt tgggttccga cgggtccggt ttgttgggaa ttgagatcga
     5641 tgaagctctc ttacgagctg ccatatctta ttgccccccg ggatcagatc tggattgaga
     5701 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     5761 ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat
     5821 ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct
     5881 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
     5941 ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat
     6001 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     6061 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     6121 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     6181 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     6241 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga
     6301 tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     6361 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6421 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6481 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     6541 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     6601 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     6661 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     6721 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     6781 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     6841 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     6901 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     6961 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7021 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7081 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7141 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7201 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7261 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7321 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7381 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7441 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7501 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7561 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     7621 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     7681 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     7741 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     7801 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     7861 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     7921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8101 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     8761 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     8821 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     8881 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8941 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9001 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9061 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     9121 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     9181 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9241 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9301 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9361 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9421 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9481 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     9541 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     9601 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     9661 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     9721 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     9781 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
     9841 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
     9901 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
     9961 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10021 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10081 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    10141 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    10201 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10261 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10321 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10381 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10441 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10501 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    10561 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    10621 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    10681 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    10741 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    10801 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    10861 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    10921 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    10981 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11041 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    11101 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    11161 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    11221 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11281 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11341 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11401 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11461 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    11521 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    11581 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    11641 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    11701 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    11761 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    11821 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    11881 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    11941 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12001 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12061 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    12121 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    12181 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12241 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12301 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12361 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12421 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12481 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    12541 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    12601 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    12661 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    12721 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    12781 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    12841 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
